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Biulding a
cDNA Library
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3:10
YouTube
Lucas Learns
cDNA Synthesis By Reverse Transcription
Normally DNA is transcribed into mRNA but this process can also be reversed. To do this we can utilize the 3’-Poly A tail which all mRNA have in common. Since this tail only contains adenosine nucleotides, we can use it as a location for our primer. We can use an Oligo (dt) Primer which contains a sequence of 12-20 thymine nucleotides ...
30.7K views
Dec 7, 2022
Watch full video
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