How to Prepare and Plate Semi-Solid Methylcellulose Medium for Cell Culture
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I get methyl cellulose in powder form from TALAS, and follow instructions from papercraftpanda’s blog to make the MC. Then it’s ready to mix with PVA. In Telluride, which is a very dry place, we used 60/40 PVA mix (60% pva 40% mc). I’m finding 70/30 better for me in Southern California, and depending on the day, it’s a bit wet so I’ll add more PVA. Be aware that this also means you need to press your books longer! I had to press my most recent journal batch for 2 nights instead of 1. If you use
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